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Primary Source For State Data Primary Source For State Data Coursework My School My School If you are looking for a specific article, In the primary source for state data coursework my school main menu, under the courses heading, you should be able to navigate to the section that includes the article. This entire Coursework coursework IAA are published for your use under a Creative Commons Non-Commercial Share Alike Attribution (CC-NC-SA) licence.Growth of low temperature-tolerant recombinant Bacillus licheniformis C3, NLBG3, in small scale high concentration of organic solvents.
The use of Bacillus licheniformis in the production of recombinant proteins requires continuous cultivation of large number of cells at higher temperature and concentration of inorganic salts. The low temperature-tolerant B. licheniformis C3 (pNLBG3:Bpe) was successfully constructed through insertion of ice nucleation protein (INP) gene in the chromosome and in the plasmid. In this study, pNLBG3:Bpe was cultivated at 30 degrees C and 96% ethanol, 70% ethanol, and 80% glycerol was successfully added up to 33% (v/v) in recombinant B. licheniformis NLBG3 (pNLBG3:Bpe) batch fermentation. The cultivation of B. licheniformis C3 (pNLBG3:Bpe) showed that the addition of 10% glycerol (v/v) in the batch fermentation resulted in higher cell concentration of 1.4 x 10(8) cfu/ml, 1.5 x 10(8) cfu/ml, and 1.9 x 10(8) cfu/ml than the control (0% glycerol). At 100% glycerol, the cell concentration of NLBG3 (pNLBG3:Bpe) was 9.2 x 10(7) cfu/ml. The result of inorganic salt sensitivity test showed that when glycerol was used at 10%, 100%, and 33% (v/v), salt concentration was more stable. However, the amount of salt concentration in glycerol-free medium was higher than that of a control medium. These results showed that the B. licheniformis C3 (p ac619d1d87
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